Generator

Part:BBa_K566024

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)

ho1 gene (constitutive expression)

ho1 gene transcribed from constitutive promoter. ho1, along with pcyA, converts heme into the chromophore phycocyanobillin (PCB).


Usage and Biology

Encoding heme oxygenase-1 (HO1). Is required for chromophore synthesis in photosynthetic light-harvesting complexes, photoreceptors, and circadian clocks. pPLPCB(S) plasmid from Tabor et al (2010) carries this gene and ho1. These two genes convert heme into phycocyanobilin (PCB) via a two-step oxidation/reduction process.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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